Assessment of fecal coliforms in Catha edulis (Khat) leaves along the value chain in Igembe South Sub-County, Meru, Kenya
DOI:
https://doi.org/10.58506/ajstss.v4i2.248Keywords:
Fecal coliforms, Sanitation Practices, Catha edulis (Khat)Abstract
Background: Catha edulis leaves have been found to contain high levels of fecal coliforms, exceeding recommended limits for safe consumption, therefore posing a high risk of spreading bacterial diseases. The presence of fecal coliforms in these leaves is a concern for consumers and those involved in their production and transportation. Therefore, this study assessed the fecal coliform contamination rate of Catha edulis (Khat) leaves at different stages of the value chain in Igembe South Sub-County, Meru, Kenya.
Materials and Methods: This study was conducted and it involved 328 respondents from 25 locations within the Sub-County, and it adopted a cross-sectional descriptive study design involving one-time sampling of Khat leaves during farming, handling, vending, and consumption. Khat leaves were collected in aerated bags, processed, and evaluated for the presence of fecal coliforms at the Meru University of Science and Technology Laboratory. Data collected were analyzed using descriptive statistics such as mean, standard deviation, minimum, and maximum and inferential statistics such as Tukey HSD with the aid of Statistical Package of Social Sciences (SPSS) version 23.0. Results: Study findings from Tukey HSD revealed that handlers had significantly higher fecal coliform contamination with mean difference=0.1162; p=.000 than vendors (mean difference=0.0994; p=.002), customers (mean difference=0.1096; p=.001) and farmers having the lowest with mean difference=0.0169; p=.000. Conclusion: The study concluded that handling was the most contributing factor to fecal coliform contamination. The study recommends the need for reduce fecal coliform contamination among handlers as well as vendors, customers and farmers by improving sanitation hygiene practices such as handwashing, proper use of sanitation facilities, sanitation of surfaces and equipment and proper storage of khat leaves.
Key words; Fecal coliforms contamination, Assessment, Sanitation practices and value chain.
References
Al-Amin, M., & Kamal, M. M (2021). Integration of MALDI TOF MS in the Most Probable Number Method for Enumeration of Escherichia coli Significantly Reduces the Assay Time.
Al-Barakani, A. (2021). Expansion of city scale, traffic modes, traffic congestion, and air pollution. Cities, 108, 102974.
Al‐Maweri, S. A., Warnakulasuriya, S., & Samran, A. (2018). Khat (Catha edulis) and its oral health effects: An updated review. Journal of investigative and clinical dentistry, 9(1), e12288.
Amare, G., Dobo, B., & Haile, E. (2023). The Effect of Wet Coffee Processing Plant Effluent on Physicochemical and Bacteriological Quality of Receiving Rivers Used by Local Community: Case of Aroresa District, Sidama, Ethiopia. Environmental Health Insights, 17, 11786302231165186.
Drechsel, P., Qadir, M., & Galibourg, D. (2022). The WHO guidelines for safe wastewater use in agriculture: a review of implementation challenges and possible solutions in the global south. Water, 14(6), 864.
Hamed, N. A. H. (2020). Occurrence of Escherichia coli and Fecal Coliforms in Drinking Water from Selected Localities in Khartoum State, Sudan (Doctoral dissertation, Sudan University of Science & Technology).
Horváth, K. M., Kocsis, T. J., Lenkovics, B., Fekete-Frojimovics, Z., & St, A. (2022) Coliform Bacteria Contamination Measurement Of Cell Phones And Users'hands. Editorial Board, 2.
Kamau, P., Aloo-Obudho, P., Kabiru, E., Ombacho, K., Langat, B., Mucheru, O., & Ireri, L. (2012). Prevalence of intestinal parasitic infections in certified food-handlers working in food establishments in the City of Nairobi, Kenya. Journal of biomedical research, 26(2), 84-89.
Nthiga, J. N. (2022). An investigation on and isolation of parasites that infect humans associated with Catha edulis leaves sold in selected parts of Nairobi County, Kenya (Doctoral dissertation, JKUAT-COHES).
Nthiga, J. N., Mbugua, A., & Anjiri, C. (2019). An investigation on and isolation of human parasites associated with contaminated catha edulis leaves sold in selected parts of Nairobi City County, Kenya. International Academic Journal of Health, Medicine and Nursing, 1(2), 132-144.
Okpala, C. O. R., & Korzeniowska, M. (2023). Understanding the relevance of quality management in agro-food product industry: From ethical considerations to assuring food hygiene quality safety standards and its associated processes. Food Reviews International, 39(4), 1879-1952.
Orr, A., Donovan, J., & Stoian, D. (2018). Smallholder value chains as complex adaptive systems: a conceptual framework. Journal of Agribusiness in Developing and Emerging Economies.
Park, S., Navratil, S., Gregory, A., Bauer, A., Srinath, I., Jun, M., ... & Ivanek, R. (2013). Generic Escherichia coli contamination of spinach at the preharvest stage: effects of farm management and environmental factors. Applied and environmental microbiology, 79(14), 4347-4358.
Ripanda, A. S., Rwiza, M. J., Nyanza, E. C., Njau, K. N., Vuai, S. A., & Machunda, R. L. (2021). A Review on contaminants of emerging concern in the environment: A focus on active chemicals in Sub-Saharan Africa. Applied Sciences, 12(1), 56.
Suleiman, T. S., Karimuribo, E. D., & Mdegela, R. H. (2018). Prevalence of bovine subclinical mastitis and antibiotic susceptibility patterns of major mastitis pathogens isolated in Unguja island of Zanzibar, Tanzania. Tropical animal health and production, 50, 259-266.
Tominaga, T. (2019). Rapid detection of coliform bacteria using a lateral flow test strip assay. Journal of microbiological methods, 160, 29-35.
Tuffa, A. C. (2019). Value chain and nutritional analyses of warqe food products in relation to post-harvest losses. Acta Universitatis Agriculturae Sueciae, (2019: 1).
Wei, Y., Li, J., Shi, D., Liu, G., Zhao, Y., & Shimaoka, T. (2017). Environmental challenges impeding the composting of biodegradable municipal solid waste: A critical review. Resources, Conservation and Recycling, 122, 51-65.
Weis, S., Schwiertz, A., Unger, M. M., Becker, A., Faßbender, K., Ratering, S., ... & Egert, M. (2019). Effect of Parkinson’s disease and related medications on the composition of the fecal bacterial microbiota. npj Parkinson's Disease, 5(1), 28.
WHO/UNICEF (2015). Progress on sanitation and drinking water: 2015 update and MDG assessment. World Health Organization.
World Health Organization. (2017). Achieving quality universal health coverage through better water, sanitation and hygiene services in health care facilities: a focus on Ethiopia.
World Health Organization. (2022). COVID-19 weekly epidemiological update, edition 115, 26 October 2022.
Yuan, T., Vadde, K. K., Tonkin, J. D., Wang, J., Lu, J., Zhang, Z., ... & Sekar, R. (2019). Urbanization impacts the physicochemical characteristics and abundance of fecal markers and bacterial pathogens in surface water. International journal of environmental research and public health, 16(10), 1739.
Zerbe, S. (2022). Global Land-Use Development Trends: Traditional Cultural Landscapes Under Threat. In Restoration of Multifunctional Cultural Landscapes: Merging Tradition and Innovation for A Sustainable Future (Pp. 129-199). Cham: Springer International Publishing.
